Acharjee, Dalia and Mandal, Santanu and Samanta, Sujan Krishna and Roy, Mangal and Kundu, Biswanath and Roy, Sukumar and Basak, Piyali and Nandi, Samit K K (2023) In Vitro and In Vivo Bone Regeneration Assessment of Titanium-Doped Waste Eggshell-Derived Hydroxyapatite in the Animal Model. ACS Biomaterials Science & Engineering, 9 (8). pp. 4673-4685. ISSN 2373-9878

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Abstract

Inthis work, a titanium-doped hydroxyapatite (HAp) scaffold wasproduced from two different sources (natural eggshell and laboratory-gradereagents) to compare the efficacy of natural and synthetic resourcesof HAp materials on new bone regeneration. This comparative studyalso reports the effect of Ti doping on the physical, mechanical,and in vitro as well as in vivo biological properties of the HAp scaffold.Pellets were prepared in the conventional powder metallurgy route,compacted, and sintered at 900 & DEG;C, showing sufficient porosityfor bony ingrowth. The physical-mechanical characterizations wereperformed by density, porosity evaluation, XRD, FTIR, SEM analysis,and hardness measurement. In vitro interactions were evaluated bybactericidal assay, hemolysis, MTT assay, and interaction with simulatedbody fluid. All categories of pellets showed absolute nonhemolyticand nontoxic character. Furthermore, significant apatite formationwas observed on the Ti-doped HAp samples in the simulated body fluidimmersion study. The developed porous pellets were implanted to assessthe bone defect healing in the femoral condyle of healthy rabbits.A 2 month study after implantation showed no marked inflammatory reactionfor any samples. Radiological analysis, histological analysis, SEManalysis, and oxytetracycline labeling studies depicted better invasionof mature osseous tissue in the pores of doped eggshell-derived HApscaffolds as compared to the undoped HAp, and laboratory-made samples.Quantification using oxytetracycline labeling depicted 59.31 & PLUSMN;1.89% new bone formation for Ti-doped eggshell HAp as compared toTi-doped pure HAp (54.41 & PLUSMN; 1.93) and other undoped samples. Histologicalstudies showed the presence of abundant osteoblastic and osteoclasticcells in Ti-doped eggshell HAp in contrast to other samples. Radiologicaland SEM data also showed similar results. The results indicated thatTi-doped biosourced HAp samples have good biocompatibility, new bone-formingability, and could be used as a bone grafting material in orthopedicsurgery.

Item Type:	Article
Subjects:	Engineering Materials
Divisions:	Bioceramics & Coating
Depositing User:	Bidhan Chaudhuri
Date Deposited:	13 Oct 2023 09:06
Last Modified:	13 Oct 2023 09:06
URI:	http://cgcri.csircentral.net/id/eprint/5621

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