Basu, Arindam and Ray, Sayantan and Saha, Suman and Chakraborty, Jui (2021) Development and Validation of RP-HPLC Method for Estimation of Methotrexate Drug Intercalated in Mg-Al Layered Double Hydroxide Nanoparticles. Transactions of the Indian Ceramic Society, 80 (2). pp. 127-134. ISSN 0371-750X

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Abstract

In the present study, pristine magnesium aluminium layered double hydroxide (Mg-Al LDH, sample A) nanoparticles were synthesized by simple co-precipitation technique followed by intercalation of methotrexate drug (MTX), their detailed physicochemical characterization and development of RP-HPLC method for estimation of MTX intercalated in Mg-Al LDH nanoparticles (sample B). The PXRD analysis showed the basal spacing of 003 plane corresponding to sample A and sample B to be 8.63 angstrom and 11.39 angstrom, respectively. Particle size distributions were measured to be 56-75 nm (D-50=66.15 nm) in case of sample A and 75-100 nm (D-50=87.41 nm) for sample B. Simple, accurate RP-HPLC method was developed for estimation of the MTX drug from sample B, on Agilent HPLC 1200 system with UV detector on L1 column. 0.02 M potassium dihydrogen phosphate solution (pH 3.0) and acetonitrile (85:15 v/v) was used as mobile phase in an isocratic elution mode at a flow rate of 1.0 mL/min at 35 degrees C with a load of 10 mu L at 307 nm. The retention time for methotrexate was at 4.44 min. The method was validated as per ICH Q2 (R1) guidelines. The method was found to be linear over a concentration range of 10-100 mu g/mL.

Item Type:	Article
Subjects:	Engineering Materials
Divisions:	Bioceramics & Coating
Depositing User:	Bidhan Chaudhuri
Date Deposited:	24 Dec 2021 06:32
Last Modified:	24 Dec 2021 06:32
URI:	http://cgcri.csircentral.net/id/eprint/5325

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