Das, Sumita and Chatterjee, Sabyasachi and Pramanik, Srikrishna and Sujatha Devi, P and Kumar, Gopinatha Suresh (2018) A new insight into the interaction of ZnO with calf thymus DNA through surface defects. Journal of Photochemistry and Photobiology B-Biology, 178. pp. 339-347. ISSN 1011-1344

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Abstract

Experimental evidences on the binding interaction of ZnO and Calf Thymus (CT) DNA using several biophysical techniques are the centre of interest of the present study. The interaction of ZnO with CT DNA has been investigated in detail by absorption spectral study, fluorescence titration, Raman analysis, zeta potential measurement, viscometric experiment along with thermal melting study and microscopic analysis. Steady-state fluorescence study revealed the quenching (48%) of the surface defect related peak intensity of ZnO on interaction with DNA. The optimized concentration of ZnO and DNA to obtain this level of quenching has been found to be 0.049 mM and 1.027 mu M, respectively. Additional fluorescence study with 8-hydroxy-5-quinoline (HQ) as a fluorescence probe for Zn2+ ruled out the dissolution effect of ZnO under the experimental conditions. DNA conjugation on the surface of ZnO was also supported by Raman study. The quantitative variation in conductivity as well as electrophoretic mobility indicated significant interaction of ZnO with the DNA molecule. Circular dichroism (CD) and viscometry titration provided clear evidence in support of the conformational retention of the DNA on interaction with ZnO. The binding interaction was found to be predominantly entropy driven in nature. The bio-physical studies presented in this paper exploring ZnO-CT DNA interaction could add a new horizon to understand the interaction between metal oxide and DNA.

Item Type:	Article
Subjects:	Electronics
Divisions:	Sensor and Actuator
Depositing User:	Bidhan Chaudhuri
Date Deposited:	25 Sep 2018 10:54
Last Modified:	25 Sep 2018 10:54
URI:	http://cgcri.csircentral.net/id/eprint/4365

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