Kundu, Biswanath and Nandi, Samit Kumar and Dasgupta, Sudip and Datta, Someswar and Mukherjee, Prasenjit and Roy, Subhasis and Singh, Aruna Kumari and Mandal, Tapan Kumar and Das, Partha and Bhattacharya, Rupnarayan and Basu, Debabrata (2011) Macro-to-micro porous special bioactive glass and ceftriaxone-sulbactam composite drug delivery system for treatment of chronic osteomyelitis: an investigation through in vitro and in vivo animal trial. Journal of Materials Science-Materials in Medicine, 22 (3). pp. 705-720. ISSN 0957-4530

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Abstract

A systematic and extensive approach incorporating in vitro and in vivo experimentation to treat chronic osteomyelitis in animal model were made using antibiotic loaded special bioactive glass porous scaffolds. After thorough characterization for porosity, distribution, surface charge, a novel drug composite were infiltrated by using vacuum infiltration and freeze-drying method which was subsequently analyzed by SEM-EDAX and studied for in vitro drug elution in PBS and SBF. Osteomyelitis in rabbit was induced by inoculation of Staphylococcus aureus and optimum drug-scaffold were checked for its efficacy over control and parenteral treated animals in terms of histopathology, radiology, in vivo drug concentration in bone and serum and implant-bone interface by SEM. It was optimized that 60P samples with 60-65% porosity (bimodal distribution of macro- to micropore) with average pore size similar to 60 mu m and higher interconnectivity, moderately high antibiotic adsorption efficiency (similar to 49%) was ideal. Results after 42 days showed antibiotic released higher than MIC against S. aureus compared to parenteral treatment (2 injections a day for 6 weeks). In vivo drug pharmacokinetics and SEM on bone-defect interface proved superiority of CFS loaded porous bioactive glass implants over parenteral group based on infection eradication and new bone formation.

Item Type:	Article
Subjects:	Processing Science
Divisions:	Bioceramics & Coating
Depositing User:	Bidhan Chaudhuri
Date Deposited:	16 Jan 2012 12:13
Last Modified:	25 Feb 2013 08:08
URI:	http://cgcri.csircentral.net/id/eprint/273

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