Kapoor, Renu and Sistla, Pavana Goury and Kumar, Jerald Mahesh and Raj, T Avinash and Srinivas, G and Chakraborty, Jui and Sinha, Mithlesh K. and Basu, Debabrata and Pande, Gopal (2010) Comparative assessment of structural and biological properties of biomimetically coated hydroxyapatite on alumina (alpha-Al(2)O(3)) and titanium (Ti-6Al-4V) alloy substrates. Journal of Biomedical Materials Research Part A, 94A (3). pp. 913-926. ISSN 1549-3296

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Previous reports have shown the use of hydroxyapatite (HAp) and related calcium phosphate coatings on metal and nonmetal substrates for preparing tissue-engineering scaffolds, especially for osteogenic differentiation. These studies have revealed that the structural properties of coated substrates are dependent significantly on the method and conditions used for coating and also whether the substrates had been modified prior to the coating. In this article, we have done a comparative evaluation of the structural features of the HAp coatings, prepared by using simulated body fluid (SBF) at 25 degrees C for various time periods, on a nonporous metal substrate titanium-aluminium-vanadium (Ti-6Al-4V) alloy and a bioinert ceramic substrate alpha-alumina (alpha-Al(2)O(3)), with and without their prior treatment with the globular protein bovine serum albumin (BSA). Our analysis of these substrates by scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier-transform infrared (FTIR) spectrometry showed significant and consistent differences in the quantitative and qualitative properties of the coatings. Interestingly, the bioactivity of these substrates in terms of supporting in vitro cell adhesion and spreading, and in vivo effects of implanted substrates, showed a predictable pattern, thus indicating that some coated substrates prepared under our conditions could be more suitable for biological/biomedical applications. (C) 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 94A: 913-926, 2010.

Item Type: Article
Uncontrolled Keywords: Tissue engineering; Bone formation; Biomimetic coatings; Biocompatibility
Subjects: Engineering Materials
Depositing User: Bidhan Chaudhuri
Date Deposited: 13 Feb 2012 10:36
Last Modified: 13 Feb 2012 10:36
URI: http://cgcri.csircentral.net/id/eprint/795

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