Roy, Payal (2024) Special Application of Bioactive Glass as Active Ingredient in Woundcare and Hemostasis. PhD thesis, CSIR CGCRI and Jadavpur University.
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Recently, bioactive glass (BG) has emerged as a promising solution for treating injuries such as diabetic and venous ulcers. BG release the therapeutic ions that helps in fibrin clot formation by aid in platelet aggregation, supporting the coagulation cascade and helping in the regeneration of soft tissue. In the Part A of this thesis, we synthesized a binary glass composition (named, AgBG)containing varying mol% of SiO2, CaO, B2O3, and Ag2O using the sol-gel technique, followed by electrospinning with an FDA-approved polymer to fabricate a fibrous matrix named ABGmnf based wound care matrix. We then conducted various material characterization techniques including XRD, FTIR, TG-DSC, FESEM, BET, angle of repose, zeta potential, solubility assessment, and mechanical property testing. Biological studies included in vitro cytocompatibility, immunofluorescence staining for cellular proliferation and morphology, 2D wound healing assays, and antibacterial experiments to estimate zone of inhibition (ZOI) and determines minimum inhibitory concentration (MIC) using gram positive and gram negative strains. The in vitro material characterizations and biological studies confirmed the successful fabrication of ABGmnf based wound care matrix, its cytocompatibility, and antibacterial activity. Subsequently, in vivo pharmacokinetic and biodistribution studies along with biocompatibility study by assessing IL-6 and TNF-α confirmed its safety profile. This was followed by in vivo wound healing assay, which exhibited fast closure of wound, and histological assessment of various vital organs. These findings suggest that compositions like AgBG as ABGmnf based wound care matrix have great potential in the wound care market and could pave the way for new directions in tissue engineering. In the Part B of the thesis, we report a unique composition of bioactive glass, 70 SiO2: (30-x-y) CaO: x.Al2O3: y.ZnO, where x=10-18 mole% and y= 0-8 mole%, exhibiting haemostatic property as well as antibacterial activity. The as-prepared glass was characterized using XRD,SEM-EDX, FTIR, BET and TG-DSC along with in vitro degradation study and biological studies e.g., cytocompatibility, haemocompatibility, in vitro thrombus formation, in vitro blood absorption capacity, blood coagulation assays (PT, aPTT), erythrocyte adhesion assay, measuring blood clotting index (BCI), in vitro antibacterial assay against S. aureus as well as invivo acute dermal toxicity followed by histopathological analysis) and in vivo hemostasis efficacy were undertaken. The novel bioactive glass composition exhibits promises to be an efficient haemostatic agent with antibacterial activity.
| Item Type: | Thesis (PhD) |
|---|---|
| Additional Information: | Supervisor: Dr. Jui Chakraborty |
| Subjects: | Glass > Glass Properties Glass |
| Divisions: | Bioceramics & Coating |
| Depositing User: | Ms Upasana Sahu |
| Date Deposited: | 01 Sep 2025 11:28 |
| Last Modified: | 01 Sep 2025 11:28 |
| URI: | http://cgcri.csircentral.net/id/eprint/5749 |
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