Sengupta, Somoshree and Khatua, Chandra and Pal, Aniruddha and Bodhak, Subhadip and Balla, V K (2020) Influence of ultrasound and magnetic field treatment time on carcinoma cell inhibition with drug carriers: an in vitro study. Ultrasound in Medicine and Biology, 46 (10). pp. 2752-2764. ISSN 0301-5629

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The influence of exposing carcinoma cells to a static magnetic field (SMF) and low-intensity pulsed ultrasound (LIPUS), for different durations (15-45 min/d), in the presence of magnetic and non-magnetic drug carriers, on their in vitro inhibition is examined. Increasing the exposure time by 15 min/d decreased the culture duration by 24 h to achieve the same level of inhibition in colon (HCT116) and hepatocellular (HepG2) cells. Cell cycle analysis revealed enhanced cellular blockage in G1 and S phases with SMF + LIPUS exposure, and exposure for 45 min/d completely suppressed the S -> G2 transition. Apoptosis of both types of cells increased with SMF + LIPUS treatment time, and HepG2 cells exhibited elevated necrosis with >30 min/d exposure. HepG2 cells also had higher amounts of reactive oxygen species (seven- to eightfold) than HCT116 cells (two- to sixfold), suggesting treatment effectiveness is cell and drug carrier dependent. The accelerated cellular activities are attributed to the enhanced internalization of drug carriers as a consequence of destabilized cellular membranes caused by the SMF + LIPUS-generated mechanical and electrical stimuli. (E-mail: (C) 2020 World Federation for Ultrasound in Medicine & Biology. All rights reserved.

Item Type: Article
Subjects: Engineering Materials
Divisions: Bioceramics & Coating
Depositing User: Bidhan Chaudhuri
Date Deposited: 27 Jan 2021 07:45
Last Modified: 27 Jan 2021 07:45

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