Satpathy, Aishwarya and Pal, Aniruddha and Sengupta, Somoshree and Das, Ankita and Hasan, Md. Mahfujul and Ratha, Itishree and Barui, Ananya and Bodhak, Subhadip (2019) Bioactive Nano-Hydroxyapatite Doped Electrospun PVA-Chitosan Composite Nanofibers for Bone Tissue Engineering Applications. Journal of the Indian Institute of Science, 99 (3, SI). pp. 289-302. ISSN 0970-4140

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Combination of bioceramics with polymers to fabricate nanofibrous scaffolds holds enormous potential for bone tissue regeneration. In this study, we aim to incorporate HAp nanoparticles in trace doping amount in PVA-chitosan nanofiber matrix to fabricate PVA-chitosan composite nanofibers with improved performance for application as a bone tissue regeneration material. The diameter of the fabricated composite nanofibrous mat is estimated as 300 ± 121 nm. Beads free nanofibers mat with uniform morphology was ascertained for all sample groups by scanning electron microscopy (SEM) and the overall composition was assessed using Fourier transform infrared spectroscopy (FTIR) and energy dispersive X-ray spectroscopy (EDX). SEM images showed a homogeneous distribution of HAp nanoparticles in the composite nanofibers matrix. Further, X-ray diffraction (XRD) was performed to determine the crystallinity of the fabricated scaffolds. Swelling behavior and hydrolytic degradation of nanofibrous mats were subsequently evaluated by immersing in PBS buffer at pH 7.4 at physiological temperature (37 °C). The biocompatibility study of nanofiber scaffolds was performed with MC3T3 cells. Significantly higher cellular viability was observed on HAp nanoparticles incorporated composite nanofibrous scaffold surface after 7 days of culture in comparison to scaffolds without HAp.

Item Type: Article
Subjects: Engineering Materials
Divisions: Bioceramics & Coating
Depositing User: Bidhan Chaudhuri
Date Deposited: 15 Nov 2019 07:42
Last Modified: 15 Nov 2019 07:42

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