Paul, Sudeep and Pal, Anindya and Choudhury, Amit Roy and Bodhak, Subhadip and Balla, Vamsi Krishna and Sinha, Arijit and Das, Mitun (2017) Effect of trace elements on the sintering effect of fish scale derived hydroxyapatite and its bioactivity. Ceramics International, 43 (17). pp. 15678-15684. ISSN 0272-8842

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Synthesis of nano-crystalline hydroxyapatite (HAp) from fish scale (Catla calla) was carried out by calcining of raw fish scale between 200 degrees C and 1200 degrees C. The synthesized powders were characterized using thermogravimetry differential thermal analysis (TGA-DTA), X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, inductively coupled plasma atomic emission spectroscopy (ICP-AES), scanning electron microscopy (SEM) and high resolution transmission electron microscopy (HRTEM). The crystallinity, crystallite size, strain and cell parameters of fish scale derived calcined powders and chemically synthesized HAp (S-HAp) powders were estimated from XRD data. The average crystallite size of fish scale derived HAp powder increased with increasing calcination temperature. The fish scale derived HAp (FSH800) and S-HAp, calcined at 800 degrees C, showed crystallite size of 30 nm and 60 nm, respectively. ICP-AES analysis revealed that the fish scale derived HAp powder calcined at 1200 degrees C (FSH1200) contain significantly high amount of magnesium and strontium ions compared to S-HAp. After sintering at 1200 degrees C for 1 h, the relative density of FSH1200 was 20% higher than that of S-HAp due to better sinterability as a result of its small crystallite size and the presence of trace elements. In vitro cytotoxicity studies performed using human osteoblast-like cells (MG63) and mouse osteoblast cell line (MC3T3-E1) demonstrated that fish scale derived HAp (FSH1200) is non-toxic.

Item Type: Article
Subjects: Engineering Materials
Divisions: Bioceramics & Coating
Depositing User: Bidhan Chaudhuri
Date Deposited: 30 Jan 2018 07:15
Last Modified: 30 Jan 2018 07:15

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