Joshi, Nidhi and Basak, Sujit and Kundu, Sangeeta and De, Goutam and Mukhopadhyay, Anindita and Chattopadhyay, Krishnananda (2015) Attenuation of the Early Events of a-Synuclein Aggregation: A Fluorescence Correlation Spectroscopy and Laser Scanning Microscopy Study in the Presence of Surface-Coated Fe3O4 Nanoparticles. Langmuir, 31 (4). pp. 1469-1478. ISSN 0743-7463

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The aggregation of a-synuclein (A-syn) has been implicated in the pathogenesis of Parkinson's disease (PD). Although the early events of aggregation and not the matured amyloid fibrils are believed to be responsible for the toxicity, it has been difficult to probe the formation of early oligomers experimentally. We studied the effect of Fe3O4 nanoparticle (NP) in the early stage of aggregation of A-syn using fluorescence correlation spectroscopy (FCS) and laser scanning microscopy. The binding between the monomeric protein and NPs was also studied using FCS at single-molecule resolution. Our data showed that the addition of bare Fe3O4 NPs accelerated the rate of early aggregation, and it did not bind the monomeric A-syn. In contrast, l-lysine (Lys)-coated Fe3O4 NPs showed strong binding with the monomeric A-syn, inhibiting the early events of aggregation. Lys-coated Fe3O4 NPs showed significantly less cell toxicity compared with bare Fe3O4 NPs and can be explored as a possible strategy to develop therapeutic application against PD. To the best of our knowledge, this report is the first example of using a small molecule to attenuate the early (and arguably the most relevant in terms of PD pathogenesis) events of A-syn aggregation.

Item Type: Article
Subjects: Microstructure and Characterization
Engineering Materials
Divisions: Nano-Structured Materials
Depositing User: Bidhan Chaudhuri
Date Deposited: 30 Dec 2015 05:51
Last Modified: 30 Dec 2015 05:51

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