Chiavaioli, F and Biswas, Palas and Trono, C and Bandyopadhyay, Somnath and Giannetti, A and Tombelli, S and Basumallick, Nandini and Dasgupta, Kamal and Baldini, F (2014) Towards sensitive label-free immunosensing by means of turn-around point long period fiber gratings. Biosensors and Bioelectronics, 60. pp. 305-310. ISSN 0956-5663

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Long period fiber gratings have been effectively used in the field of biochemical sensing since a few years. Compared to other well-known label-free optical approaches, long period gratings (LPGs) take advantage of the typical peculiarity of optical fibers. Coupling the propagating core mode with a high-order cladding mode near its turn-around point (TAP) was the strategy adopted to achieve good performances without additional coatings, except for the sensing and selective biolayer deposited on the fiber. Both the modeling and manufacturing of TAP LPGs were discussed. After the functionalization of the fiber surface with the deposition of a Eudragit L100 copolymer layer followed by immunoglobulin G (IgG) covalent immobilization, an IgG/anti-IgG bioassay was implemented along the grating region and the kinetics of antibody/antigen interaction was analyzed. A quantitative comparison between a TAP LPG and a non-TAP LPG was carried out to highlight the improvement of the proposed immunosensor. The real effectiveness and feasibility of an LPG-based biosensor were demonstrated by using a complex matrix consisting of human serum, which also confirmed the specificity of the assay, and a limit of detection of 70 mu g L-1 (460 pM) was achieved. (C) 2014 Elsevier B.V. All rights reserved.

Item Type: Article
Subjects: Processing Science
Divisions: Fiber Optics and Photonics
Depositing User: Bidhan Chaudhuri
Date Deposited: 17 Nov 2014 09:52
Last Modified: 25 Nov 2014 10:27

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