Joshi, Nidhi and Mukhopadhyay, Anindita and Basak, Sujit and De, Goutam and Chattopadhyay, Krishnananda (2013) Surface coating rescues proteins from magnetite nanoparticle induced damage. Particle & Particle Systems Characterization, 30 (8). pp. 683-694. ISSN 0934-0866

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The presence of magnetic nanoparticles (NPs) in physiological systems induces toxicity through its effects on mitochondrial function and reactive oxygen species (ROS) imbalance. Magnetic NP induced cytotoxicity has been elaborately evaluated for impending threats, however, a detailed investigation is lacking. It is shown that the interaction of Fe3O4 NPs with cytochrome c can lead to different events based on the NPs to protein ratio, the solution conditions, and the type of surface protection. At low NPs concentration, rapid binding and subsequent electron transfer are the preferred events while at higher concentration slow oxidative modification of the protein is initiated. The slow event of protein modification yields conformational disorientation, loss of stability, and formation of amyloid-like structures with cytochrome c. The possibility that the NP induced oxidative stress and age can work in concert to compromise different aspects of cellular quality control processes is discussed. Suitable surface modifications of the NPs inhibit their direct binding to the protein molecules and minimize NP induced toxicity.

Item Type: Article
Subjects: Crystal Chemistry, Thermodynamics, Phase Equilibria
Divisions: Nano-Structured Materials
Depositing User: Bidhan Chaudhuri
Date Deposited: 06 Feb 2014 08:29
Last Modified: 06 Feb 2014 08:29

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